Non-Enzymatic, Template-Free Polymerization of 3’,5’ Cyclic Guanosine Monophosphate on Mineral Surfaces

Dr. Judit E. Šponer, Prof. Jiří Šponer, Jakub Výravský, Dr. Ondrej Šedo, Prof. Zbyněk Zdráhal, Dr. Giovanna Costanzo, Prof. Ernesto Di Mauro, Sreekar Wunnava, Prof. Dieter Braun, Dr. Roman Matyášek, Dr. Aleš Kovařík

ChemSystemsChem https://doi.org/10.1002/syst.202100017

Abstract

Previous studies on the polymerization of 3’,5’ cyclic guanosine monophosphate (cGMP) demonstrated the potential of the compound in the abiotic generation of the first oligonucleotide sequences on the early Earth. These experiments were conducted under idealized laboratory conditions, which logically raises the question whether the same chemistry could take place in the harsh environment present on our planet in its earliest days. In the current study, we focus on the mineralogical context of this chemistry and show that numerous, but not all, common minerals assumed to be present on the early Earth could host the polymerization of H-form 3’,5’ cGMP. In particular, we have found that quartz varieties are especially suitable for this purpose, similar to andalusite, amphibole or micas. On the contrary, olivine, calcite, and serpentine-group minerals interfere with the studied polymerization chemistry. Our results show that crystallization on mineral surfaces, which is mainly a diffusion controlled process, determines the ability of 3’,5’ cGMP to polymerize. The observation that numerous amorphous and crystalline SiO₂ forms are compatible with the oligomerization chemistry suggests that the process could commonly occur in a wide range of primordial environments allowing for crystallization of the cyclic monomers from a dropping solution.